RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts

RNA–protein interactions play a role in various cellular processes, and methods to study these are expanding.RNA–protein complexes can be isolated, after which bound proteins identified by western blot or mass spectrometry.Despite the variety of existing methods, it remains challenging characterize global interactome specific RNA transcript their native context upon perturbations.Promising technologies use RNA-targeting systems coupled with proximity-dependent biotinylation identify vivo. an important numerous processes health disease. In recent years, RNA-bound proteome has been extensively studied, uncovering many previously unknown RNA-binding proteins. However, little is known about particular bind transcript. this review, we provide overview interactions, focus on strategies that insights into transcripts. Finally, discuss challenges future directions, including potential CRISPR-RNA targeting investigate endogenous interactions. 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